Omic methodologies for assessing metal(-loid)s-host-microbiota interplay: A review

Omic methodologies have become key analytical tools in a wide number of research topics such as systems biology, environmental analysis, biomedicine or food analysis. They are especially useful when they combined providing new perspective and holistic view the problem. Methodologies for microbiota analysis been mostly focused on genome sequencing. However, information provided by these metagenomic studies is limited to identification presence genes, taxa their inferred functionality. To achieve deeper knowledge microbial functionality health disease, dysbiosis conditions related metal metalloid exposure, introduction additional meta -omic approaches including metabolomics, metallomics, metatranscriptomics metaproteomics results essential. The possible impact metals metalloids gut effects gut-brain axis (GBA) only begin be figured out. this end workflows combining powerful claimed high resolution mass spectrometry heteroatom-tagged proteomics absolute quantification metal-containing biomolecules using “tag” sensitive selective detector ( e.g. ICP-MS). This review focus current with techniques procedures available metallomics special attention advantages drawbacks. • influence species microbiota-brain has pointed mother-offspring transference important seeding infant gut. hypothalamic-pituitary-thyroid can also affected pollution. chemical form metal(loid) determines toxicity essentiality. assessing metal(loid)s host reviewed.